Quest diagnostics blood collection stopperlabel color. The potential for crosscontamination of additives among evacuated blood tubes has led to the development of the order of draw. Obtain appropriate medical attention in the event of any exposure. Specimen labeling requirements all specimens submitted to the laboratory must be individually labeled.
Phlebotomy order of draw below is the correct order of draw per the clsi venipuncture standard gp41 note. Vacutainer tube guide order of draw for routine volumes see separate guide for microtainers tubesbottles tube label abbrev. A phlebotomist must be familiar with all the types of equipment in order to select appropriate collection devices for the type and condition of the patients vein and the type and amount of specimen required for the test. Hematology for eg, cbc, hgb, hct, smear slide, esr, sickle cell, thallasemia. Order of draw the most current clinical and laboratory standards institute clsi guidelines recommends a single order of draw, whether using a multisample, evacuated tube system or drawing with a syringes. Find this pin and more on the phlebotomy shop by marketlab, inc phlebotomy order of draw. Order of draw blood collection tubes must be filled in a specific order to avoid specimen contamination from the additive in the preceding tube. Blue tube for coagulation sodium citrate red no gel. Blood culture vials are to be collected first in order of draw before any blood tubes.
Tube guide and recommended order of draw oxford university. Specimen collection, order of draw lwiss020 prepared by. Blood collection tubes must be filled in a specific sequence to minimize contamination of sterile specimens, avoid possible test result error caused by carryover. Discard tube 3 ml no additive must be used only if drawing a citrate blue tube with a draw royal blue top tubes in the order indicated by the additive.
Order of draw for multiple tube phlebotomy phlebotomy. Using a butterfly scalp vein needle only, add 810 mls blood to. Cap colors 810 hematology immunohematology molecular diagnostics viral markers k 3. Tube guide recommended order of draw clinical labs. Tube chart venous blood collection tubes also available in pediatric or low draw volumes of 2ml or less. When collecting multiple specimens, blood tubes should be drawn in the following order. Use the chart below to quiz yourself on the proper order of draw. Order of draw phlebotomy chapter 7 quiz proprofs quiz. Tubes tube guide and order of draw mix by color closure inversions lavender additive k 2 edta inverting 10x laboratory use for whole blood hematology determinations. Vacutainer tube guide order of draw for routine volumes. If using serum trace element tube with clot activator please collect after serum tubes use 2 identifier for patient identification. When drawing blood from a patient you are expected to know which order to follow because a mix up may give mixed. Weve compiled the above information in an easytounderstand infographic for you to easily save or share.
Skin puncture blood collection order of draw pxsc460 order of draw and order of transfer attachment 2 gts skin puncture order of draw rev 1. Vacutainer tube guide order of draw for routine volumes see. Read, sign and date document updates to acknowledge understanding of changes or amendments to the order of draw policy and forward acknowledgement to manager. Order of draw bottlestopper additive for other blood, serum or plasma separation instructions, see the specimen collection and handling section of this directory quest diagnostics specimen collection tubes when using a winged blood collection set for venipuncture and a coagulation citrate tube is the first specimen to be drawn, a discard. Order of draw in situations of multisampling, it is recommended to arrange the tubes in the following order tube volume determinations instructions fa plus 410851 microbiology to exclude the possibility of septicaemia 1 set of blood culture bottles. A phlebotomist must be familiar with all the types of equipment in order to select appropriate collection devices for the type and condition of the patients vein and the. Some tests require whole blood, plasma or serum for testing. A trained phlebotomist using the correct order of draw ensures they obtain a quality sample to be used for diagnostic purposes to provide accurate results.
Multiple tube collections blood culture bottles to be drawn if required before blood tubes trace element. Today, the gold standard in sample collection is the bd vacutainer product family, which u. Green mint green lithium heparin inversions lithium heparin and gel for in plasma inversionsseparation 10x 10x for plasma determinations in chemistry. Order of draw clsi recommended order of draw reproduced with permission from clsi. Blood culture tubes are usually at the top of the order of draw to avoid contamination of the blood collected. The following order of draw is an accepted laboratory standard. Taking these factors seriously can lessen discomfort and complications for your patient, lessen procedure time, and prevent errors in diagnosis caused by cross contamination of additives between blood collection tubes. Specimen labels may be ordered from laboratory services please call client services. Additive number of inversions at blood collection do not shake general laboratory use volume draw blclt 1 aerobic and 1 anaerobic culture bottles 5 blood culture. Additive number of inversions at blood collection do not shake general laboratory use volume draw blclt 1 aerobic and 1 anaerobic culture bottles 5 blood culture requires adapter. Draw policy to avoid cross contamination of additives between tubes.
Bd vacutainer order of draw for multiple tube collections. Tube guide order of draw special instructions two patient identi. Gp41a6, procedures for the collection of diagnostic. Phlebotomy tubes and order of draw university of iowa phlebotomy tubes and order of draw. All plastic tubes must be gently inverted 510 times to provide thorough mixing of. Blood collection tubes order of draw calgary lab services. Evidence supporting the need for a specific order in which blood collection tubes should be filled was first published over 30 years ago, yet the concept remains elusive to many healthcare professionals with sample collection responsibilities. Bd vacutainer order of draw when drawing blood directly or transferring from a syringe into vacutainer tubes, it is extremely important to follow the specified order of draw. Specimens must be collected in the appropriate collection container, kit or device, correctly labeled. Therefore, if a coagulation specimen is drawn first, a discard tube a no additive or coagulation tube is recommended to be drawn prior to this tube to ensure the proper anticoagulanttoblood ratio. Test tube guide and order of draw test tube alternate test tube additive vol. Container labeling must match information on requisition. Tube content and volume test list blood culture bottles adult green and orange paed yellow.
Start with quality specimen collection to achieve better results. Skin puncture blood collection tubes and cards, which are most often used for infant and pediatric collections, must be collected in a specific sequence to reduce the effect of. If drawn out of order, test results can be significantly different or incorrect. For more than 60 years, bd life sciences preanalytical systems has advanced the science of specimen collection to help laboratory tests become the foundation for 70% of all medical decisions. Order of draw for multiple tube collections when collecting multiple specimens, blood tubes should be drawn in the following order. When a winged blood collection set butterfly is used and a citrate tube is first to be drawn, a discard nonadditive tube should be used to. Draw order tube topstopper color tube type 1 blood cultures 2 light blue citrate 3 redgoldtigerserum 4 greenheparin 5 lavenderedta 6 grayoxalate here is a mnemonic device to help you remember. The collection tubes contain different types of additives, which are specific for the individual tests.
Order of draw is the tube sequence a certified phlebotomist needs to follow while collecting blood. Label containers do not put labels on caps or lids. Common blood collection tubes, their additives and. Order of draw order of draw clsi recommended gp41 if a winged blood collection set is used, the first tube in the series will be underfilled. Draw order tube topstopper color tube type 1 blood cultures 2 light blue citrate 3 redgoldtiger serum 4 green heparin 5 lavender oxalate edta 6 gray. Specimen collection, order of draw austin pathology. Tubes must be collected in the order indicated according to the tube additive in order to prevent contamination or interference from a preceding tube to a following tube. Order of draw for multiple tube collections designed for your safety handle all biologic samples and blood collection sharps lancets, needles, luer adapters and blood collection sets according to the policies and procedures of your facility. This is because bacteria from nonsterile tube stoppers or shields may result in bacteria growth, which will show in the test result as a blood infection. Blood collection tubes order of draw pxsc460 order of draw and order of transfer attach 1 rev 1.
Gp41a6, procedures for the collection of diagnostic blood specimens by venipuncture. Aug 29, 2018 all questions 5 questions 6 questions 7 questions 8 questions. Order of draw special instructions two patient identi. Aerobic followed by anaerobic if insufficient blood for both culture bottles use aerobic bottle. Order of draw for multiple tube collections number. Venous blood collection order of draw interior health. Some tubes have either liquid or powdered anticoagulants or powdered glass.
Hollman tube type common tests refer to austin pathology test directory instructions. The order of draw is designed to eliminate the possibility of cross contamination that may result in erroneous results. Order of draw of blood collection tubes the procedure follows guidelines set forth by the clinical and laboratory standards institute formerly known as nccls in order to insure that quality specimens are collected for laboratory testing. Quality manager page 1 of 1 general enquiriesresults enquiries phone labtests. Blood collection equipment, additives, and order of draw. Order of draw is a topic that the content instructor teaches first, but most students struggle with it, so plan this lesson when students need additional support, before inclass practicals or off campus clinicals. Gold sst plain tube wgel and clot activator additive.
Blood collection tube guide order of draw this document is controlled in printed form approver approved. Blood collection order of draw mixing chart separation clot spin pour into transport vial label with specimen type and patient id for other blood, serum or plasma separation instructions, see the. For clot to form, keep 10 15 minutes tube at room temperature for minimum 30 mins. K potassium edta, ppts plasma preparation tubes specimen. Click the showhide all button to hide the tube color and descriptions. The following orderofdraw is recommended when drawing several specimens during a single. Vacutainer tube guide order of draw for routine volumes see separate guide for microtainers tubes bottles tube label abbrev.
No additive red therapeutic drug monitoring toxicology. Phlebotomy guidelines and order of draw specimen collection procedures proper specimen collection and handling is a critical part of obtaining a valid laboratory result. The sequence below is the order the evacuated tubes must be. When drawing blood for multiple tube collections, it is important to take into consideration proper venipuncture technique and correct order of draw. Phlebotomy order of draw explained e phlebotomy training. Atkinson version 5, issued sept 2018 page 1 of 1 approved by. Phlebotomy order of draw and study aid phlebotomy coach. Specimens must be collected in the appropriate collection container, kit or device, correctly labeled and transported promptly to the laboratory. Order of draw bottlestopper additive for other blood, serum or plasma separation instructions, see the specimen collection and handling section of this directory quest diagnostics specimen collection tubes when using a winged blood collection set for venipuncture and a coagulation citrate tube is the.
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